Wheat is an annual grass plant. It is restricted due to the scarcity of salt-tolerant germplasm resources. In particular, the salt-tolerance variation of wheat is very limited and the genetic basis is narrow, which seriously restricts the research and utilization of salt-tolerance in wheat. Therefore, conducting research on the genetic diversity of wheat salt tolerance, broadening the genetic basis, and screening new salt-tolerant genes are of great theoretical value and practical significance for developing and utilizing salinized soil, expanding wheat planting area, and increasing unit yield. Randomly amplified DNA (RAPD) molecular markers can detect genetic differences across the entire genome at the molecular level. They have many marker sites and are not affected by environmental conditions. Therefore, they have been widely used in many aspects.
Run 50 or 100 PCR reactions with slightly different DNA samples or primers in each tube. It is necessary to first prepare a mixed reaction custom genetic solution of all reagents with different amounts of components, and then aliquot them into each tube. Since agarose gel is required for verification, the final volume of the reaction is 20 µL.
RAPD Analysis of Genetic Diversity for Salt Tolerance in Wheat